Journal: Discover Oncology

Article Title: Penicilazaphilone C triggers ferroptosis in triple-negative breast cancer cells via the MDM2/p53/SLC7 A11/GPX4 pathway

doi: 10.1007/s12672-025-02687-w

Figure Lengend Snippet: PAC induces ferroptosis by activating the MDM2/p53/SLC7 A11/GPX4 signaling axis. A Analysis from the STRING database reveals interactions between MDM2 and p53, as well as between SLC7 A11 and GPX4. B Western blot analysis revealed that treatment with PAC decreased the protein levels of MDM2, SLC7 A11, and GPX4, while concurrently increasing the levels of p53. C Overexpression of MDM2 led to a decrease in p53 expression. D CCK-8 assays demonstrated a reduction in cell viability upon PAC treatment, which was partially restored by overexpression of MDM2, large SD reflects biological variability and differences in transfection efficiency. Flow cytometry analysis showed an increase in lipid peroxides ( E ) and Fe 2+ ( F ) following PAC treatment, while a significant decrease in expression occurred with MDM2 overexpression. This trend was reversed by PAC treatment. The data are presented as the mean ± standard deviation of five independent replicates and were analyzed using a two-way ANOVA, followed by post hoc multiple comparisons. Identical letters above columns denote nonsignificant differences, whereas distinct letters indicate significant differences with a p-value of less than 0.05

Article Snippet: Hoechst nuclear dye ( H10325 ) was sourced from Thermo Fisher Scientific (Waltham, Massachusetts, USA).The mouse monoclonal anti-p53 antibody (DO-7, GA61661-2, Dako, Denmark) and the mouse monoclonal anti-MDM2 antibody (SMP14, Santa Cruz Biotechnology) were unconjugated and detected using species-specific secondary antibodies conjugated to horseradish peroxidase (HRP) or fluorophores, depending on the assay.

Techniques: Western Blot, Over Expression, Expressing, CCK-8 Assay, Transfection, Flow Cytometry, Standard Deviation

Journal: Discover Oncology

Article Title: Penicilazaphilone C triggers ferroptosis in triple-negative breast cancer cells via the MDM2/p53/SLC7 A11/GPX4 pathway

doi: 10.1007/s12672-025-02687-w

Figure Lengend Snippet: PAC inhibits the development of TNBC tumors and promotes ferroptosis in vivo. After a 28-day post-inoculation period in nude mouse models injected with MDA-MB-231 and MDA-MB-436 cells, images illustrating the tumor volume ( A, B ) were obtained, along with precise quantitative assessments of the tumor volumes in the control and PAC treatment groups ( C ). Flow cytometry analysis showed an increase in lipid peroxides ( D ) and Fe 2+ ( E ) after PAC treatment. F The expression of MDM2, p53, SLC7 A11, and GPX4 was analyzed by Western blot in tumor tissues. The data are presented as the mean ± standard deviation of five independent experiments, each with two technical replicates, and were analyzed using a t-test. Significant differences are indicated by asterisks: ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05. Non-significant differences are denoted by “ns” (p > 0.05)

Article Snippet: Hoechst nuclear dye ( H10325 ) was sourced from Thermo Fisher Scientific (Waltham, Massachusetts, USA).The mouse monoclonal anti-p53 antibody (DO-7, GA61661-2, Dako, Denmark) and the mouse monoclonal anti-MDM2 antibody (SMP14, Santa Cruz Biotechnology) were unconjugated and detected using species-specific secondary antibodies conjugated to horseradish peroxidase (HRP) or fluorophores, depending on the assay.

Techniques: In Vivo, Injection, Control, Flow Cytometry, Expressing, Western Blot, Standard Deviation